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. Author manuscript; available in PMC: 2016 Apr 20.
Published in final edited form as: Dev Cell. 2015 Apr 9;33(2):125–135. doi: 10.1016/j.devcel.2015.02.021

Figure 4. The BMP-Smad4, not TGFβ-Smad4, signaling cascade regulates Sox2+ dental epithelial stem cell maintenance during molar development.

Figure 4

(A) Timing of doxycycline induction and sample harvest in control, KRT14-rtTA;tetO-Cre;Bmpr1afl/fl (K;T;B) and KRT14-rtTA;tetO-Cre;Tgfbr2fl/fl (K;T;T) mice. (B, C) Macroscopic views of control compared to KRT14-rtTA;tetO-Cre;Bmpr1afl/fl lower first molars (B) and control compared to KRT14-rtTA;tetO-Cre;Tgfbr2fl/fl lower first molars (C) at PN48.5. (D-G) H&E staining of control compared to KRT14-rtTA;tetO-Cre;Bmpr1afl/fl lower first molars (D, F) and control compared to KRT14-rtTA;tetO-Cre;Tgfbr2fl/fl lower first molars (E, G) at PN7.5. Red arrows indicate the HERS. Red broken line in (F) indicates the cervical loop. (H-K) In situ hybridization of Amelogenin in control compared to KRT14-rtTA;tetO-Cre;Bmpr1afl/fl lower first molars (H, I) and control compared to KRT14-rtTA;tetO-Cre;Tgfbr2fl/fl lower first molars (J, K) at PN7.5. Black arrows indicate Amelogenin expression, whereas arrowhead indicates lack of expression. (L-O) Immunofluorescence of Sox2 (green) in control compared to KRT14-rtTA;tetO-Cre;Bmpr1afl/fl lower first molars (L, M) and control compared to KRT14-rtTA;tetO-Cre;Tgfbr2fl/fl lower first molars (N, O) at PN7.5. White arrow indicates expression, whereas white arrowheads indicate absence of expression. Scale bars: 50μm.