Table 3.

Inflammatory gene expression changes between infarcted (saline or MMP-12i groups) and no MI (day 0 controls) as well as between MMP-12 inhibitor treated and vehicle control groups (MMP-12i vs saline). The increase, decrease, and no change were based on quantitative measurements that were statistically different based on RT²-PCR analysis for n=6 samples/ group. Qualitative directions of change and not average Ct values for each group are shown here. Out of 84 genes measured, 45 genes at d1 and 41 genes at d7 were significantly different among groups (all p<0.05).

	Saline vs Day 0	MMP-12i vs Day 0	MMP-12i vs Saline
Day 1 post-MI
(a): Il10, Il11, Tgfb1 (p): Ccl9, Ccl20, Ccr8, Cd40lg, Cxcl1, Cxcl10, Il3, Lta, Xcr1 (both): Il4	↑
(a): Il1r2, (p): Ccl2, Ccl3, Ccl4, Ccl7, Ccr1, Cxcl5, Il1b, Il17b, Il1r1, Il18rb, Il20, Il1f6, Il1f8, Il2rg, Itgam, Spp1	↑	↑
(p): Ccl25	↓
(p): Cxcl11, Il2rb		↓
(a): Abcf1, Bcl6, Il10rb, Tollip (p): C3, Ccl11, Ccr10, Cxcl9, Il15, Il6st, Mif, Scye1	↓	↓
Day 7 post-MI
(p): Cxcl10	↑
(a) Il4, Tgfb1 (p) Casp1, Ccl3, Ccl4, Ccl9, Ccl17, Ccr2, Ccr3, Il18, Il1f6, Il1f8, Il20, Lta		↑
(a): Il1r2 (p) Ccl20, Ccr1, Cd40lg, Cx3cl1, Il2rg, Il3, Itgb2, Itgam, Spp1, Xcr1	↑	↑
(p): Il6ra		↑	↑
(a): Il11 (p): Cxcr5, Il1r1	↑	↑	↑
(p): Pf4, Tnf	↓
(a): Abcf1, Tollip (p): Ccl11, Cxcl9, Ccr10, Il15, Il6st, Mif, Scye1	↓	↓

Arrows depict direction of change; n=6/group. (a)- anti-inflammatory; d-day; MI- Myocardial infarction; (p)-pro-inflammatory. The genes discussed in manuscript are shown in bold.

Gene description in alphabetical order: Abcf1 – ATP-binding cassette, sub-family F (GCN20) member 1, Bcl – B-cell leukemia/lymphoma 6, C3 – Complement component 3, Casp1 – Caspase 1, Ccl – Chemokine (C-C) ligand, Ccr – Chemokine (C-C) receptor, Cd40lg – Cd40 ligand, Cx3cl – Chemokine (C-X3-C motif) ligand 1, Cxcl – Chemokine (C-X-C) ligand, Cxcr – Chemokine (C-X-C motif) receptor 3, Il – Interleukin, Il10rb – Interleukin 10 receptor beta, Il1f - Interleukin 1 family, member, Il1r – Interleukin 1 receptor type I, Il1r2 – Interleukin 1 receptor type I, Il2rb – Interleukin 2 receptor beta chain, Il2rg – Interleukin 2 receptor gamma chain, Il6st – Interleukin 6 signal transducer, Itgam – Integrin alpha M, Itgb – Integrin beta 2, Lta – Lymphotoxin, Mif – Macrophage migration inhibitory factor, Pf4 – Platelet factor 4, Scye1 – Small inducible cytokine subfamily E member 1, Spp1 – Osteopontin, Tgfb – Transforming growth factor beta, Tnf – Tumor necrosis factor, Tollip – Toll interacting protein, Xcr1 – Chemokine (C motif) receptor 1