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. Author manuscript; available in PMC: 2016 Apr 21.
Published in final edited form as: Anal Chem. 2015 Apr 10;87(8):4572–4577. doi: 10.1021/acs.analchem.5b00789

Figure 2.

Figure 2

Analysis of the E. coli proteome digest using on-line pH gradient elution from a SCX SPE-CZE system. Experimental conditions: 50 μm i.d.×150 μm o.d.×1.5 cm sulfonate SCX SPE column; 50 μm i.d.×150 μm o.d.×60 cm long LPA coated capillary as separation capillary; 50 ng E. coli digests loading amount; 90 nL of 30 mM elution buffers at each elution step; 50 mM FA separation buffer; 19.5 kV separation voltage; and 1.5 kV spray voltage.