Fig. 8.

KYL-conjugated protamine increases the uptake of siRNA in LNCaP cells. Two and a half microliters of the BLOCK-iT™ Fluorescent siRNA (20 μM) were mixed with 1.5 μg protamine (or the protamine-KYL conjugate) in 100 μl serum-free RPMI-1640 medium at room temperature for 10 min to form the complex. After washing cells with PBS, the siRNA complexes were added to each well at a final concentration of 200 nM. Five hours after the transfection, the fluorescence was examined using a Leica DMI3000 fluorescence microscope.