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. 2015 Jan 28;20(3):451–460. doi: 10.1007/s12192-015-0570-7

Table 1.

Primers used in cloning of rice sHSPs

sHSP and KOME accession number Vector and construct name Additional residues from vector Primersa
Peroxisome-Hsp16
AK105317 		pET-28a(+)
Peroxisome-Hsp16-tag		 23 F: AGACCATGGCGGACCTCTTCTTC
R: GCGAAGCTTTTATCAGAGCTTGCTGGAGAC
Cytoplasm-Hsp16.9
AK121025 		pET-28a(+)
Cytoplasm-Hsp16.9-tag		 23 F: ATAGCTAGCATGTCGCTGGTGAG
R: GCGGATCCTTAGCCAGAAATCTC
pET-21b
Cytoplasm-Hsp16.9-tagless		 2 F: GCTCATATGTCGCTGGTGAGGC
R: GCGGATCCTTAGCCAGAAATCTC
Nuclear-Hsp18.6
AK119261 		pET-28a(+)
Nuclear-Hsp18.6-tagA		 23 F: GGAGCTAGCATGACGGAGCTGTTC
R: ATTGGATCCTCAGGCGATGGTGAC
pPROEX HTa
Nuclear-Hsp18.6-tagB		 24 F: ATCCATGGCAACGGAGCTGTTCGAC
R: GCGGAATTCTCAGGCGATGGTGAC
Hsp24
AK105464 		pPROEX HTa
Mitochondria-Hsp24		 24 F: ATACCATGGGCGGGTCCCGTGCA
R: GCCGAATTCCTACTCGACGTTGACC
Hsp26.7
AK063618 		pRSET C
Chloroplast-Hsp26.7-Tag		 14 F: CGAGCTAGCCAGGAGAACAGGGACAAC
R: ATTGGATCCCTACTGGACCTGCACGTC
pRSET A
Chloroplast-Hsp26.7-tagless		 1 F: CGCCATATGCAGGAGAACAGGGACAAC
R: ATTGGATCCCTACTGGACCTGCACGTC

F forward, R reverse primer

aRestriction sites are underlined