Table 1.
Gene transcripts, primer sequence, and resulting fragment size
| Gene | Sequence of nucleotide (5′–3′) | Fragment size (bp) | Annealing temp (°C) | NCBI/reference |
|---|---|---|---|---|
| MCT1 | F: ATGGGCATCAACTACCGACTTC R: CTCTTTGGGGCTTCCTTCTATG |
168 | 55 | EU404088.1 |
| MCT2 | F: CAAGCCTGGTGGTATATGC R: CAAGAAGAACTGGGCAACAC |
153 | 60 | EU650275.1 |
| MCT4 | F: CCCGTGTTCGTGGTGAGCTA R: TGAAGAGGTAGACGGAGTAA |
160 | 58 | EU650276.1 |
| HSP27 | F: AGGAGCGGCAGGATGAG R: GGACAGGGAGGAGGAGAC |
101 | 60 | Kamanga-Sollo et al. (2011) |
| HSP70 | F: GTGGCTCTACCCGCATCCC R: GCACAGCAGCACCATAGGC |
114 | 62 | Kamanga-Sollo et al. (2011) |
| HSP90 | F: CGCTGAGAAAGTGACCGTTATC R: ACCTTTGTTCCACGACCCATAG |
126 | 60 | Kamanga-Sollo et al. (2011) |
| RPS15a | F: AATGGTGCGCATGAATGTC R: GACTTTGGAGCACGGCCTAA |
100 | 60 | XM_005679050.1 |
EMBL accession number or reference of published sequence