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Multiple sequence alignment of the identified heat shock factor sequences. Buffalo HSF-1, HSF-2, HSF-4 and HSF-5 protein sequences were aligned by using cluatalW2 program in BioEdit software. The secondary structure of HSF protein sequence is shown above the sequence. Arrow underneath the alignment indicates domain boundaries. Black and blue box depicts NLS and NES, respectively. Dot represents the conserved residues in the protein sequences
