Phenotypes of Hybrid Aspen Ectopically Overexpressing PttCLE41 and/or PttPXY Genes
(A) Sections from tissue-culture-grown plantlets 3 weeks post-rooting. Where two images are shown in the upper panel, they were selected to show the range of phenotypes observed. Scale bars represent 200 μM (upper panels) and 50 μM (lower panels). Red asterisks show examples of organized files of cells. The xylem (x) and phloem (ph) are indicated.
(B) Representative greenhouse-grown plants 3 months after transfer to soil.
(C) Phenotypes of hybrid aspen with targeted overexpression of PttCLE41 and PttPXY. Left-hand panels show sections from tissue-culture-grown plantlets 3 weeks post-rooting while greenhouse-grown plants 3 months after transfer to soil are shown on the right. Scale bars represent 200 μM. The xylem (x) and phloem (ph) are also indicated. Arrows highlight the disrupted xylem. See also Figure S1.