FIG 1.

Growth curves of G. sulfurreducens grown on NBAF at 30°C for 168 h in 100-ml serum bottles (red line) and in a 5-liter bioreactor with (green line) and without (blue line) nicotinamide supplementation. Relative peak areas (normalized to an internal standard) of nicotinamide in the medium (purple line) detected by GC-MS in the 5-liter bioreactor supplementation experiment are plotted against the sampling time. The time point measurements for the serum bottles are means of three biological replicates with error bars indicating the standard deviation. Single measurements were recorded for the bioreactor samples due to volume constraint.