TABLE 2.
L. lactis strain | CWPS type | Efficiency of plaque formationb |
|||
---|---|---|---|---|---|
WRP3 | L47 | 949 | P087 | ||
3107 | C2 | 1 | 1 | 1 | 1 |
NZ9000 | C1 | ≤2.87 × 10−8 | ≤1.83 × 10−7 | ≤4.20 × 10−6 | ≤2.51 × 10−6 |
NZ9000/pPTPi (induced) | C1 | ≤2.87 × 10−8 | ≤1.83 × 10−7 | ≤4.20 × 10−6 | ≤2.51 × 10−6 |
NZ9000::GT1/pPTPi (induced) | C1 | ≤2.87 × 10−8 | ≤1.83 × 10−7 | ≤4.20 × 10−6 | ≤2.51 × 10−6 |
NZ9000::GT1/pPTPi-C2 (uninduced) | C2 | ≤2.87 × 10−8 | ≤1.83 × 10−7 | ≤4.20 × 10−6 | ≤2.51 × 10−6 |
NZ9000::GT1/pPTPi-C2 (induced) | C2 | 7.8 × 10−2 | 1.01 × 10−1 | 3.9 × 10−2 | 6.08 × 10−2 |
Host range swapping ability is defined as the ability of phages that originally infected the lactococcal C2-type CWPS strain 3107 and were unable to infect the C1-type lactococcal strain NZ9000 to infect a derivative of NZ9000 that expresses the C2-type CWPS in place of its native C1-type CWPS.
The efficiencies of plaque formation of the phages on each strain are expressed relative to that on the original host, 3107. All results are averages for assays performed at least in triplicate.