FIG 5.

S. aureus PNAG production and eDNA presence versus adhesion forces. (A and C) Normalized PNAG production in 24-h-old S. aureus ATCC 12600 (A) and S. aureus ATCC 12600 Δpbp4 (C) biofilms as a function of the adhesion force. (B and D) Normalized eDNA presence in 24-h-old S. aureus ATCC 12600 (B) and S. aureus ATCC 12600 Δpbp4 (D) biofilms as a function of the adhesion force. Linear regression analysis was performed for all graphs to analyze the correlation between PNAG production, eDNA presence, and adhesion force. The solid lines represent the best fit to a linear function, while r² values represent the correlation coefficients. The dotted lines enclose the 95% confidence intervals. The amounts of PNAG and eDNA were normalized to the amount of biofilm formed on each substratum, and each point represents the average ± standard error of the mean from three individual experiments with separately grown staphylococcal cultures. AU, arbitrary units.