TABLE 1.
Primers used for RT-qPCR analyses and qPCR calibration curve parameters
| Primer | Sequence | Target gene (locus tag) | Amplicon length (bp) | Slope | y intercept | Amplification efficiency (%) | R2 | Reference |
|---|---|---|---|---|---|---|---|---|
| Slo_nirK853f | AAGGTGGGTGAGTCTGTGCT | nirK (Shew_3335) | 188 | −3.393 | 35.64 | 97.1 | 0.999 | 23 |
| Slo_nirK1040r | GGCTGGCGGAAGGTGTAT | |||||||
| Slo_nrfA1083f | GGATATCCGTCACGCTCAAT | nrfA (Shew_0844) | 226 | −3.299 | 34.93 | 101.0 | 0.992 | 24 |
| Slo_nrfA1308r | GTCCATACCCAATGCAGCTT | |||||||
| Slo_16Sf | CACACTGGGACTGAGACACG | 16S rRNA genes | 191 | −3.461 | 34.27 | 94.5 | 1.000 | 23 |
| Slo_16Sr | TGCTTCTTCTGCGAGTAACG | |||||||
| luc_refA | TACAACACCCCAACATCTTCGA | Luciferase gene control mRNA | 67 | −3.247 | 35.46 | 103.2 | 0.999 | 29 |
| luc_refB | GGAAGTTCACCGGCGTCAT |