Schematic of the embryo and particle fluctuation data. (A) Schematic drawing of the syncytial blastoderm and the injection geometries we used. Inset: detailed cortical organization of nuclei, microtubules, and F-actin. (B) Fluorescence image of beads (red) dispersed within the nuclear layer (green). Scale bar = 5 μm. (C) Intensity map of bead 4 with its center of mass indicated by an arrow. The red trajectory shows the bead movement determined with subpixel resolution at a rate of 16,000 frames/s over ∼93,000 frames (pixel size: 331 nm). (D) Position power spectral densities in x direction of all four beads shown in the fluorescence image. (E) Mean squared displacements in x direction of all four beads shown in the image. Power-law slopes are drawn for comparison. To see this figure in color, go online.