(a) Surface pressure-area isotherms (π-A isotherms) of CL/DPPC mixed monolayers at various ΦCL, ranging from 0 to 100 mol %, at an air-water interface. The isotherms were recorded at a compression rate of 10 cm2/min with total area of 30 × 20 cm2. The temperature was monitored and maintained at 25°C. (b) Variations in molecular areas A with ΦCL for mixed CL/DPPC monolayers at 3 (before LE-LC coexistence), 7 (after LE-LC coexistence), and 10, 20, 30, and 40 mN/m were calculated from the π-A isotherms to understand the expanding effect of CL on mixed monolayers at different packing states.