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. 2014 Dec 20;16:504. doi: 10.1186/s13058-014-0504-9

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© Verbeke et al.; licensee BioMed Central 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Invasive tumor formation 6 and 8 weeks after intraductal injection of 4G-shp53-PI3K tumor cells. A. Mammary ducts dilated by human tumor cells at 6 weeks. B. Diffuse blue masses 8 weeks after intraductal injection caused by tumor cells invading the stroma. C. Mixed DCIS and invasive tumor at 8 weeks. H&E stain and immunohistochemistry show increased keratin 14 staining as cells migrate from the ducts into the stroma. Early squamous changes are marked by arrows. There are small difference in the structures in the serial sections in C because the structures gradually change as the sectioning proceeds through the paraffin block. Scale bars A 1 mm, B 2 mm, C 100 μm.