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. 2014 Dec 20;16:504. doi: 10.1186/s13058-014-0504-9

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© Verbeke et al.; licensee BioMed Central 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Replacement of the murine luminal layer 6 weeks after intraductal injection of 4G-shp53 cells. A. Stereomicrograph showing spread of human cells within the ducts. Alternating yellow and green areas in the right panel demonstrate independent engraftment of multiple clones. B&C. Histopathology (H&E stain) and immunohistochemistry show that the human cells have replaced the murine luminal layer with morphologically normal human cells. The human cells are larger, as seen at junctions between murine and human luminal cells. GFP, keratin 7 and keratin 19 staining specifically label the human cells. The cells are ERα+, PGR-, Ki67+ luminal cells. SMA staining shows that the myoepithelial layer is intact. D. GATA3 and FOXA1 are positive in murine ducts (marked by arrows) but negative in humanized ducts (marked by an arrowhead). Scale bars A 1 mm, B 100 μm, C 50 μm, D 200 μm.