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. 2014 Dec 20;16:504. doi: 10.1186/s13058-014-0504-9

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© Verbeke et al.; licensee BioMed Central 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The myoepithelial layer in DCIS is murine in origin. Ducts containing DCIS 3 weeks after intraductal injection of 4G-shp53-PI3K tumor cells were costained for p63 (red), GFP (green) and DNA (DAPI, blue) (n = 4). The p63-positive cells do not stain for GFP, indicating that they are murine in origin. Red arrow, normal murine duct; green arrow, DCIS. Scale bars 50 μm.