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. 2015 Apr 18;13:33. doi: 10.1186/s12958-015-0031-2

Table 1.

Primer sequences for various transcripts used in the study

Primer Sequence Annealing temperature
Sca-1
F AGAGGAAGTTTTATCTGTGCAGCCC 66°C
R TCCACAATAACTGCTGCCTCCTGA
Gfra
F GGCCTACTCGGGACTGATTGG 58°C
R GGGAGGAGCAGCCATTGATTT
Prohibitin
F GTGGCGTACAGGACATTGTG 58°C
R AGCTCTCGCTGGGTAATCAA
Protamine
F GGCCACCACCACCACAGACACAGGCG 66°C
R TTAGTGATGGTGCCTCCTACATTTCC
Dazl
F GTGTGTCGAAGGGCTATGGAT 61°C
R ACAGGCAGCTGATATCCAGTG
PCNA
F 5′ GATGCCGTCGGGTGAATTTG 3′ 55°C
R 5′ TCTCTATGGTTACCGCCTCCT 3′
SCP3
F 5′ TGTTGCAGCAGTGGGAACTGGAT 3′ 68°C
R 5′ CCATCTCTTGCTGCTGAGTTTCCA 3′
18s
F 5′ GTCCCGTAGACAAAATGGTGA 3′ 58°C
R 5′ TGCATTGCTGACAATCTTGAG 3′
Gapdh
F GTCCCGTAGACAAAATGGTGA 58°C
R TGCATTGCTGACAATCTTGAG