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. 2015 Apr 19;15:106. doi: 10.1186/s12870-015-0485-6

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© Foley et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Western blot of 5 ug protein extracts from various lupin samples. The blots were probed using antibodies generated against ALPHA conglutin (expected size 56 kD), BETA conglutin (68-72 kD), GAMMA conglutin (49 kD – uncleaved, 33 kD cleaved), and DELTA conglutin (18 kD– uncleaved, 12 kD cleaved) peptides, and the commercially available PEPC antibody to illustrate similar protein loading. A) Protein expression of conglutins in L. angustifolius Tanjil and wheat flour. B) Protein expression in developmental stages of L. angustifolius Tanjil seeds. C) Protein expression of conglutin in mature seeds of various lupin species/varietie.