Table 2.
Effect of different concentrations of caffeine, pentoxifylline, and kallikrein on the hyperactivation of frozen bovine spermatozoa.
| Treatments | Concentrations | Progressive | Slowly | Dead |
|---|---|---|---|---|
| Control | 0.0 | 55.0 ± 0.8c | 23.0 ± 0.8d | 22.0 ± 0.8de |
|
| ||||
| Caffeine | 1 mM | 55.2 ± 0.7c | 26.2 ± 0.4a | 18.7 ± 0.4f |
| 5 mM | 61.5 ± 0.8a | 24.7 ± 1.1bc | 13.8 ± 0.4h | |
| 10 mM | 49.8 ± 0.7f | 25.0 ± 0.7b | 25.2 ± 0.7c | |
|
| ||||
| Pentoxifylline | 1 mM | 60.2 ± 1.2b | 23.7 ± 0.4d | 16.2 ± 0.8g |
| 5 mM | 60.0 ± 0.7b | 23.8 ± 0.4cd | 16.2 ± 0.8g | |
| 10 mM | 49.8 ± 1.2f | 21.3 ± 0.4e | 28.8 ± 0.8b | |
|
| ||||
| Kallikrein | 1 U/mL | 51.3 ± 0.4e | 26.2 ± 0.4a | 22.5 ± 0.5d |
| 4 U/mL | 53.7 ± 0.4d | 25.0 ± 0.7b | 21.3 ± 0.4e | |
| 8 U/mL | 00.0 ± 0.0g | 6.3 ± 0.4f | 93.7 ± 0.4a | |
Mean values in the same columns with different superscripts (a, b, c, d, e, f, g, and h) differ significantly (P ≤ 0.05).