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. 2014 Dec 13;15(1):1102. doi: 10.1186/1471-2164-15-1102

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© Wang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of unigene expression as Reads Per kb per Million (RPKM). Expression level was determined using the RPKM method, thereby eliminating the influences of gene length and sequencing discrepancies on the calculation of gene expression. A, RPKM with 102,857 unigenes, B, RPKM after selection based on FDR ≤ 0.001 AND |log₂Ratio| ≥ 1with 3,977 unigenes. Va-24 hr: RPKM at 24 hours after infection with V. alginolyticus, TSB (tryptic soy broth)-24 hr: RPKM at 24 hours after injection with TSB.