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. 2014 Dec 13;15(1):1102. doi: 10.1186/1471-2164-15-1102

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© Wang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Complement-related pathway gene expression in controls and grouper challenged with 1.3 × 10 ⁶ CFU/ml (20 μl/fish) Vibrio alginolyticus , as determined by qRT-PCR. ( A ) A2M, ( B ) BF, ( C ) CR2, ( D ) C1q, ( E ) C1r, ( F ) C1s, ( G ) C3, ( H ) C4, ( I ) C4BP, ( J ) C5, ( K ) C6, and ( L ) C6-d. Values are presented as the mean ± SEM (n = 5). Values with different letters differ significantly. Significance was set at P < 0.05, as determined by one-way ANOVA followed by Duncan’s test.