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. 2015 Apr 23;6:321. doi: 10.3389/fmicb.2015.00321

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Copyright © 2015 Beaume, Köhler, Fontana, Tognon, Renzoni and van Delden.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Selection of Pseudomonas aeruginosa mutants affected in competition with bacterial pathogens. (A) P. aeruginosa Tn-mutants were spotted on Staphylococcus aureus bacterial lawn on MH-agar plate. This panel shows an example of a mutant displaying an altered inhibition zone compared to the others, indicating that this mutant is not able to compete with S. aureus. (B) P. aeruginosa Tn-mutants were spotted on LB agar plate with or without Klebsiella pneumoniae. The unbroken line shows a mutant drastically affected by the presence of K. pneumoniae whereas it had a normal growth on plate without competitor.