Table 2.

Primers used in this study

Primers	Amplified gene(s)	Sequences (5′–3′)	Final concentration (μM)
Semirandom PCR
ARB1		GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT	0.5
ARB2		GGCCACGCGTCGACTAGTAC	0.4
MCL195		GATCCCGCAGTGGCTCTCTATACAAAGTTG	0.2
MCL210		TGGTGCTGACCCCGGATGAAG	0.4
Confirmation of the transposon insertion site and complementations∗
PurF-Kpn	purF	ACAGGTACCAGTGATTTTGGCGGGACAC	0.6
PurF-BamHI		ACAGGATCCCCAGGGTGTCGAAGGCC	0.6
BioA-Kpn	bioA	ACAGGTACCTGAACACCCCCAACATGAGA	0.6
BioA-R_BamHI		ACAGGATCCGATTCGAGGGTAGTGGCGAC	0.6
CarB-F_BamHI	carB	ACAGGATCCGTTCGTCGATCCCGGCTA	0.6
CarB-R_HindIII		ACAAAGCTTTCGGCGTTTTCCTTGAGG	0.6
pqsC-Xba	pqsCDE	ACACTCTAGATTCGAACTGGCGTCGCAAC	0.6
pqsC-Hind		ACACAAGCTTTCTTCCAGTCGATAGCCAACC	0.6
lasA deletion∗
LasAF-Eco	5′ lasA region	CCCGGAATTCAGGATAACGTCGGCATGGAC	0.6
LasAR-Bam		CCGCGGATCCTGCTCCAGGTATTCGCTCTTG	0.6
LasAF-Bam	3′ lasA region	CCGCGGATCCACCAGATCCAGGTGAGCAACG	0.6
LasAR-Hind		CCCCAAGCTTTCGGAGTCCGGCTACTACGC	0.6

^∗Restriction enzyme recognition sites are underlined