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DATS affected on the levels of up-signals proteins associated with migration and invasion in HT-29 cells. Cells were treated with 3.12 μM of DATS for 0, 6, 12, 24 and 48 hrs. The total proteins were collected and the proteins levels iNOS, COX II, uPA (A); Ras, Rho A, FAK and SOS (B); PI3-K, MKK7 and MEKK3 (C); ERK1/2, JNK1/2 and p38 (D) were examined by Western blot as described in Materials and methods.
