Fig 4.

Representative double-labelled immunofluorescence images. Nuclei are counterstained with DAPI (blue). Images of c-kit negative were not shown here; scale bar = 20 μm. (A) CD34 (red) in moniliform appearance cells overlying vimentin (green) cells in sham control (three small solid arrows), confirmed the presence of telocytes (TCs) and telopodes (Tps) around capillaries, immunophenotype is CD34 (+)/vimentin (+)/c-kit (−). Two large dotted arrows indicated CD34 (+) capillary cells. (B and C) The number of CD34 (+)/vimentin (+) cells with typical TCs morphology and well-defined DAPI nuclei, decreased significantly, scarce or even undetectable (P = 0.000) in EMs-affected oviduct. *P < 0.05 versus sham control. Error bars = SD. (For the sake of clarity, only half of the error bars are shown).