Fig 1.

HSP27 overexpression-mediated gemcitabine sensitivity through early S-phase arrest and consequent apoptosis. Flow cytometry comparing cell cycle distribution and sub-G1 cell fractions upon gemcitabine treatment at 0, 15 or 25 nM for 24 or 48 hrs between parental PL5 cells and their HSP27-overexpressing counterparts PL5/hu18: Histograms of representative cell cycle distributions (A and C) and statistical analyses from at least three independent experiments (B and D). Error bars represent SEM. (E) Representative microscopic pictures (magnification, ×10 and ×100) displaying typical morphological features of chromatin condensation and nuclear fragmentation upon gemcitabine at 0, 15 or 25 nM in PL5/hu18 cells (inserts and arrows).