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. 2014 Oct 14;19(2):371–382. doi: 10.1111/jcmm.12449

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© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

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Fig 2 — Influence of PDGFBB inhibition on cervical cancer cells (A) migration: Ca Ski (left) and HeLa (right) cells were fluorescently labelled, plated in the top chamber of cell culture inserts, treated with negative control- and PDGFBB-siRNA and allowed to migrate towards different stimulators for 24 and 48 hrs, (B) invasion: Ca Ski and HeLa cells were treated with negative control- and PDGFBB-siRNA for 48 hrs on 12 well plates, harvested and plated on the top chamber of Matrigel pre-treated cell culture inserts and allowed to invade for 24 hrs. (a and b) negative control- and PDGFBB siRNA-treated Ca Ski cells; (c and d) negative control- and PDGFBB siRNA-treated HeLa cells microscopic visualization of invaded cells.