Figure 2.

Claudin-4 surface localization changes over time in piglet jejunum. Claudin-4 is localized to the apical aspect of the surface of jejunal intestinal enterocytes at 24 hours of age ((a), (b)) and at 48 hours of age ((c), (d)). At 3 ((e), (f)) and 5 ((g), (h)) days of age, Claudin-4 is localized along the cellular surface between the adjacent cells and no longer at the apical aspect of the enterocytes. At 6 weeks of age, there is very strong expression at the lateral membranes between adjacent enterocytes ((i), (j)). These images are representatives of IHC performed on tissue from 3 animals per time point. Primary antibody: rabbit anti-Claudin-4. Secondary antibody: FITC-labeled goat anti-rabbit (green). Nuclear stain: DAPI (blue).