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. 2015 Feb 19;21(5):452–465. doi: 10.1093/molehr/gav007

Figure 5.

Figure 5

CLDN4 and FUT4 mRNA and protein expressions in HTR8/SVneo cells. (a) CLDN4 and FUT4 mRNA expressions were reduced by small interfering RNA (siRNA) transfection as measured by qPCR. *P < 0.001; (b and c) CLDN4 and FUT4 protein expressions were reduced by siRNA transfection as measured by western blot (b) and immunocytochemistry staining (c). CLDN4 displayed a primary membrane staining, and FUT4 was a primary cytoplasmic staining. Blue color showed the 4′,6-Diamidino-2-phenylindole (DAPI) nuclear staining; Red color was CLDN4 or FUT4 protein staining. Magnification of images: ×400.