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. Author manuscript; available in PMC: 2015 Jul 29.
Published in final edited form as: J Mol Biol. 2014 May 29;426(15):2755–2768. doi: 10.1016/j.jmb.2014.05.011

Figure 2. Comparing binding affinities of NHERF1 for ezFERM, mFERM, full-length Merlin(wt), and Merlin(S518D), respectively.

Figure 2

Figure 2

(A) SPR experiments were performed with NHERF1 immobilized on a CM5 sensor chip. Merlin constructs and ezFERM at different concentrations were flown on the NHERF1 immobilized sensor chip. (B) ITC experiments to determine the binding of NHERF1 to ezFERM, mFERM, Merlin(wt), and Melrin(S518D). The binding results are summarized in Table 2.