Fig. 3.

GW4064 upregulates CYP2B6 expression via FXR. (A) Primary human hepatocytes from three different donors were treated with vehicle (DMSO) or GW4064 (1 μM) for 48 hours in triplicate. RNA was collected, and CYP2B6 mRNA expression level was measured by using qRT-PCR. (B) HepG2 cells were cotransfected with pGL3-CYP2B6 and Renilla vector and treated with vehicle or GW4064 (1 μM), and dual luciferase assay was performed. (C) HEK293T cells were cotransfected with pGL3-CYP2B6 (or pGL2-SHP) and Renilla vector, along with FXR and RXR expression vectors. On the next day, the cells were treated with vehicle (DMSO) or GW4064 (1 μM). The cell lysates were collected after 24 hours, and dual luciferase assays were performed. **P < 0.01; ***P < 0.001.