TABLE 1 .

G protein localization in the kidney

Summary of the vascular and nephron segments that have been identified for Gα subunits in the kidney. Techniques used to determine the presence of the Gα subunits are indicated with lettered footnotes.

Gα Subunit	Glomerulus	Proximal Convoluted Tubule	Thick Ascending Limb	Collecting Duct	Preglomerular Blood Vessels
Gs	+a,b	+a,c	+c,d	+c,d	+b
Gi1	+b	−c	−d, +c	−d	+b,d
Gi2	+b,c	−c	+d, −c	+c,d	+b
Gi3	+b,c	+c	−d, +c	−d	+b
Go	+b	N/D	−d	−d	N/D
Gq	+b	− (+ through its interaction with GPCR)	−d	−d	+b
G11	+b	N/D	−d	+d	+b
G14	N/D	N/D	−d	+d	N/D
G15/16	N/D	N/D	N/D	N/D	N/D
G12	+c	+c	+c	+c	+c
G13	−c	+c	−c	+c	+c

+, positive; −, negative; N/D, not determined.

^aIn situ hybridization.

^bWestern blot analysis of isolated segments.

^cImmunohistochemistry.

^dMicrodissection of renal tubular segments followed by RT-PCR.