Fig. 3.

CB2 stimulation by JWH-133 decreases M1 while increasing M2 phenotype activation of macrophages in vitro. (A) Thioglycollate-induced peritoneal macrophages were treated with JWH-133 (25 and 5 µM, shown as J25 and J5), and 1 hour later, cells were stimulated with LPS (100 ng/ml). Conditioned media from macrophage cultures were analyzed for proinflammatory cytokines (TNF-α, IL-12, and anti-inflammatory cytokine IL-10) by enzyme-linked immunosorbent assay. Peritoneal macrophages were treated with JWH-133 (5 µM) for 1 hour and then stimulated toward M1 or M2 phenotypes. (B) IL-10 secretion was quantified in conditioned medium from M1-polarized macrophages using enzyme-linked immunosorbent assay. RNA isolated from the macrophage cultures was used for quantitative PCR to quantify relative expression of genes: Arg-1 (C) and Chi3l3 (D). *P < 0.05; ** P < 0.01. Veh, vehicle.