Figure 5. Degradation of cross-linked recombinant human tropoelastin clusters by pseudolysin.

Tropoelastin (10 mg/ml) was cross-linked with 10 mM BS³ in 10 mM PBS for 30 min at 37°C (a and e). The cross-linked product was incubated with 0.1 mg/ml pseudolysin for 2 h (b and f), 3 h (c and g) and 4 h (d and h). e-h are the same samples as a-d, examined at a higher magnification as indicated by the scale bar. Scale bars in a-d: 1 μm. Scale bars in e-h: 200 nm.