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. 2015 May;87(5):842–854. doi: 10.1124/mol.114.096743

Fig. 10.

Fig. 10.

Expression of luciferase in EL4 cells in the presence or absence of Fas UTR containing the miR-23a binding site or FasL UTR containing the miR-18b binding site post-VEH or post-DES treatment. EL4 cells were transfected with pmiRGLO without an insert (MOCK), pmiRGLO–Fas UTR, pmiRGLO–Fas UTR + mature miR-23a, pmiRGLO–FasL UTR, or pmiRGLO–FasL UTR + mature miR-18b. EL4 cells were also transfected with a vector containing green fluorescent protein (GFP). Forty-eight hours post-transfection, cells were examined for GFP expression by flow cytometry for transfection efficiency. (A) Luciferase assays were performed to determine luciferase expression in EL4 cells post-transfection. (B) Luciferase data are presented as a fold change in expression. Data are depicted as mean ± S.E.M. of three independent experiments. The asterisk (*) in (B) indicates a statistically significant (P < 0.05) difference between the groups compared.