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ZMC1 treatment in complete media increases intracellular [Zn2+]free. (A) Structures of compounds used in this study and their Kd values for Zn2+. Kd values shown are from Yu et al. (2014). (B) Imaging of intracellular Zn2+ level in complete media. HEK293 and TOV112D cells were loaded with FZ3-AM followed by 1 μM of the indicated treatment in 0.2% DMSO for 20 minutes at 37°C and imaged using a 20× (NA = 0.75) air objective. Scale bar = 100 μm.
