Figure 3.

Exposure of HT29 cells to 5 μM arsenic trioxide for 48 hours significantly reduced Gli1, Gli2, CCND1, and Bcl2 mRNA expression.
Notes: Treatment of HT29 cells with 0.5 μg/mL recombinant human Shh significantly increased Gli1, Gli2, CCND1, and Bcl2 mRNA expression (n=3, *P<0.05, **P<0.01).
Abbreviations: mRNA, messenger RNA; PCR, polymerase chain reaction; Shh, Sonic Hedgehog.