Figure 4. A) Immunoblotting showing reverse expression dynamics of HPV oncoprotein E6 and host p53 proteins in curcumin-treated cancer cells. Oncoprotein E6 decreased but host p53 increased with the increasing concentration of curcumin. GAPDH was utilised as loading control for protein samples. B) Northern blotting of HPV16/E6 and β-actin mRNA to detect their mRNA transcript levels. Decreased expression of E6 mRNAs seen post-curcumin treatment. Re-probing filters with β-actin cDNA probes confirmed equal loading of mRNA in each lane.