Fig. 2.

Traditional OR-PAM of LV in the skin of a mouse ear and tail. (a) PAM image of a mouse ear at 595 nm before the injection of EB, showing only blood vessels. (b) PAM images of the same mouse ear at 560 and 595 nm after the injection of 3% EB in the ear pinnae. (c) Spectral separation of the blood vessels (shown in red) and LV (shown in blue) in the ear skin. BV, blood vessels; LV, lymphatic vessels. (d) PAM image of a mouse tail vasculature at 560 nm before the EB injection. (e) PAM of the oxygen saturation of hemoglobin (${\mathrm{sO}}_2$) in the mouse tail, where the tail veins and arteries can be separated. (f) Spectral separation of blood vessels and LV in the tail after the EB injection.