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. 2015 Apr 24;16:24. doi: 10.1186/s12865-015-0093-0

Figure 2.

Figure 2

Effects of OGF or LDN on splenic lymphocytes from EAE mice. Lymphocytes isolated from spleens of C57BL/6 mice immunized with MOG35–55 and injected daily with 10 mg/kg OGF (OGF), 0.1 mg/kg naltrexone (LDN) or 0.1 ml saline (saline); normal mice received saline at each injection point. Splenocytes from 3–4 mice per treatment at 5 and 8 days were isolated, labeled, and sorted by flow cytometry. Values represent means ± SEM. Significantly different from normal mice at p < 0.05 (*) or p < 0.001 (***), and from saline-treated immunized mice at p < 0.05 (+) or p < 0.01 (++).