Figure 4.

Inhibition of proliferation of T lymphocyte subpopulations in left inguinal nodes in EAE mice following OGF or LDN treatment. Total lymphocytes isolated from the left inguinal nodes of mice immunized with MOG_35–55 and injected daily with 10 mg/kg OGF (OGF), 0.1 mg/kg naltrexone (LDN), or 0.1 ml saline (saline); normal mice received saline at each injection point. The left inguinal node was considered “draining” on day 12, 5 days following injection on the left flank. Lymphocytes from 3–4 mice per treatment at 5 and 12 days were isolated, labeled, and sorted by flow cytometry. Values represent means ± SEM. Significantly different from normal mice at p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***), and from saline-treated immunized mice at p < 0.05 (+).