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. 2015 Mar 19;14:62. doi: 10.1186/s12943-015-0329-x

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© Galenkamp et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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TNFα primes for FasL-induced cell death and upregulates Fas expression. A. SK-N-AS cells were left untreated or were pre-treated for 24 h with 100 ng/ml TNFα or 100 ng/ml Fc:hFasL and subsequently washed and left untreated (UT) or treated for 24 h with 100 ng/ml TNFα or 100 ng/ml Fc:hFasL. Cell viability was assessed by calcein AM staining. B. SK-N-AS cells were treated or not with 100 ng/ml TNFα for the indicated times and mRNA levels of the indicated genes were assessed by qRT-PCR. mRNA expression levels were normalized using 18S. C. Western blot of Fas expression in SK-N-AS cells treated with 100 ng/ml TNFα for the indicated times. α-Tubulin was used as loading control. ***p ≤ 0.001.