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. Author manuscript; available in PMC: 2015 Apr 23.
Published in final edited form as: Mol Reprod Dev. 2015 Feb 20;82(3):251–264. doi: 10.1002/mrd.22468

Figure 5.

Figure 5

SMAD 2/3 phosphorylation in BCB-screened, GV-stage oocytes. Samples of control, BCB+ and BCB− GV stage oocytes (n=6 replicates of 20 oocytes/group) were subjected to Western blot analysis of total (t)-SMAD2/3 (A), phosphorylated (p)-SMAD2/3 (B). Expression levels were normalized relative to abundance of endogenous control (actin). Phosphorylation level (C) was expressed as p -SMAD2/3 / t-SMAD2/3 Data are shown as mean ± standard error. Values with different superscripts across treatments indicate significant differences (P < 0.05). Representative Western blot (D).