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. Author manuscript; available in PMC: 2015 Apr 23.
Published in final edited form as: Mol Reprod Dev. 2015 Feb 20;82(3):251–264. doi: 10.1002/mrd.22468

Figure 7.

Figure 7

Effects of exogenous follistatin supplementation on indices of development for embryos derived from BCB-screened, GV-stage oocytes. Presumptive zygotes derived from in vitro fertilization of oocytes from control, BCB+ and BCB− groups were cultured with or without 10 ng/ml follistatin (n=25-30 presumptive zygotes/group; n = 4 replicates) for 72 hr, then cultured in fresh medium (minus FST) until d 7. Effect of exogenous follistatin on (A) proportion of embryos that reached the 2-cell stage within 30 h post fertilization (early cleaving), (B) total cleavage rate (determined 48 hpi), (C) proportion of embryos developing to the 8-16 cell stage (72 hpi) and (D) proportion of embryos developing to the blastocyst stage (d 7). Data are shown as mean ± standard error. Values with different superscripts across treatments indicate significant differences (P < 0.05).