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. Author manuscript; available in PMC: 2015 Oct 13.
Published in final edited form as: Nat Commun. 2015 Apr 13;6:6752. doi: 10.1038/ncomms7752

Figure 2. ATF3 increases the Tip60 HAT activity.

Figure 2

(a) ATF3 increased the Tip60 HAT activity. The Tip60 HAT activity was determined by incubating 0.5 μg of GST-Tip60 with 5 μl of [3H]-acetyl CoA, 0.5 μg of histone H4, and/or 1 μg of recombinant ATF3. The data are presented as mean ±s.e.m. (n=3). (b) ATF3 promoted Tip60 autoacetylation. 0.5 μg of GST-Tip60 were incubated with H4, [3H]-acetyl CoA, ATF3 (0.5, 0.75, 1 μg), or BSA (1 μg) as indicated, and subjected to SDS-PAGE followed by autography.