Table 1.
Skeletal muscle mitochondrial proteomics
| Gene | MCK-PGC1α/NT | P |
|---|---|---|
| Housekeeping | ||
| VDAC | 1.31 | 0.024–0.128* |
| β-Oxidation | ||
| Acaa2 | 3.81 | 0.002 |
| Acadl | 3.37 | 0.004 |
| Adacm | 2.42 | 0.003 |
| Acads | 2.54 | 0.010 |
| Acadyl | 3.23 | 0.003 |
| Acot13 | 4.31 | 0.000 |
| Acsl1 | 2.61 | 0.010 |
| Cd36 | 2.46 | 0.001 |
| Cpt1b | 2.38 | 0.005 |
| Crat | 3.33 | 0.003 |
| Cpt2 | 3.33 | 0.009 |
| Dci | 2.39 | 0.000 |
| Decr1 | 2.20 | 0.001 |
| Ech1 | 2.70 | 0.007 |
| Etfa | 2.59 | 0.003 |
| Etfb | 2.66 | 0.009 |
| Fabp3 | 3.00 | 0.000 |
| Hadh | 2.56 | 0.001 |
| Hadha | 2.60 | 0.006 |
| Hadhb | 2.73 | 0.003 |
| Slc25a20 | 3.09 | 0.001 |
| Antioxidant | ||
| Aldh2 | 1.79 | 0.004 |
| Hspa9 | 1.93 | 0.006 |
| Phb | 2.05 | 0.004 |
| Phb2 | 1.85 | 0.030 |
| Prdx3 | 1.80 | 0.020 |
| Prdx5 | 3.57 | 0.004 |
| Sod2 | 2.79 | 0.001 |
| TCA cycle | ||
| Abcd3 | 2.17 | 0.012 |
| Aco2 | 2.04 | 0.002 |
| Cs | 2.13 | 0.008 |
| Dlat | 1.76 | 0.009 |
| Dld | 1.78 | 0.010 |
| Dlst | 2.02 | 0.019 |
| Etfdh | 2.19 | 0.002 |
| Fh | 2.03 | 0.017 |
| Idh3b | 1.79 | 0.012 |
| Mdh1 | 4.14 | 0.013 |
| Mdh2 | 2.29 | 0.013 |
| Ogdh | 1.97 | 0.021 |
| Pdha1 | 1.90 | 0.004 |
| Pdhb | 1.78 | 0.031 |
| Sdha | 1.91 | 0.001 |
| Sdhb | 1.86 | 0.006 |
| Sdhc | 1.86 | 0.000 |
| Sucla2 | 2.17 | 0.018 |
| Suclg1 | 2.22 | 0.025 |
| Tufm | 2.09 | 0.019 |
| Ckmt2 | 3.32 | 0.011 |
| Got2 | 3.19 | 0.007 |
| Aldh2 | 1.79 | 0.004 |
| Hspa9 | 1.93 | 0.006 |
| Phb | 2.05 | 0.004 |
| Phb2 | 1.85 | 0.03 |
| Energy and amino acid metabolism | ||
| Ckmt2 | 3.32 | 0.011 |
| Got2 | 3.19 | 0.007 |
| Slc25a3 | 5.69 | 0.004 |
Mitochondria were isolated from the quadriceps muscles of MCK-PGC1α and NT mice fed an HFD for 6 weeks. Protein abundance was analyzed by a triple quadrupole mass spectrometer using a targeted assay and normalized to a BSA standard. VDAC was measured as a housekeeping protein, and the range of P values from four separate assays is provided.
*Expression levels of VDAC were significant (P < 0.05) in only one of the four assays performed. Data are expressed as a ratio of protein abundance measured in MCK-PGC1α relative to NT mice. Statistical significance was analyzed by Student t test. n = 3–4 per group.