Figure 5.

Laminin and collagen IV are increased in diabetic (DM)-Sema3a⁺ glomerular nodules. A: Dual-fluorescent immunohistochemistry shows increased laminin (green) and collagen IV (red) colocalized to glomeruli from diabetic Sema3a⁺ gain-of-function mice (DM-Sema3a⁺ + doxycycline [dox]). Quantitation of immunoreactive laminin (B) and collagen IV (C) signals demonstrates a significant increase in glomeruli from diabetic Sema3a⁺ gain-of-function mice. D: Western blotting shows decreased total kidney laminin in diabetic Sema3a⁺gain-of-function kidneys. E: MMP-2 and -9 are downregulated in Sema3a⁺ gain-of-function diabetic kidneys. F–H: Renal VEGF-A signaling is not upregulated in diabetic mice with Sema3a⁺ gain of function. F: Plasma VEGF-A is elevated in all diabetic mice compared with nondiabetic mice, irrespective of Sema3a⁺ gain-of-function induction. G: Kidney VEGF-A protein expression measured by ELISA is downregulated in diabetic mice with Sema3a⁺ gain of function. H: VEGFR2 kidney protein expression is decreased in diabetic mice with Sema3a⁺ gain of function. *P < 0.05 vs. corresponding control. #P < 0.05 vs. nondiabetic control.