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. 2015 Apr 21;15:34. doi: 10.1186/s12894-015-0030-9

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© Hypolite et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of PKC inhibition by Bim-1 on carbachol dose-response curve. A, Raw tracers of contractile responses to increasing concentrations of carbachol in the control (top panel) strips, and strips pre-treated with Bim-1 (bottom panel). B, Bim-1 (28 nM) had no significant effect on either the sensitivity to muscarinic stimulation (EC 50), or the maximum contraction (PF) upon carbachol application (p = 0.061 for all concentrations of carbachol).