Fig 1. Characterisation of NSDV core proteins in infected cells.

(A) Vero cells were infected with the NSDVi isolate at a MOI of 5 TCID₅₀ (NSDV) or left uninfected (uninf.). After 16 h, cells were harvested, lysed in sample buffer and proteins separated by SDS-PAGE; proteins were detected by Western blot using sera raised against the NSDV N protein, the C-terminus of the L protein or the N-terminus of the L protein, as indicated. (B) Sheep kidney epithelial cells (PO) or Vero cells were infected with the NSDVi isolate at a MOI of 0.3 TCID₅₀. After 16 h, cells were fixed using 4% PFA followed by ice cold methanol, and immunolabelled using sera raised against the NSDV N- or the C-terminus of the L protein followed by AlexaFluor-568 goat anti-rabbit IgG (red). DAPI was used as a counterstain (blue). Bars indicate 20 μm.